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Edlyn at MPI-CBG Postdoc Retreat

Edlyn attended the 7th Annual MPI-CBG Postdoc Retreat, which took place in the beautiful and picturesque Tisá, Czech Republic, from 19th to 21st of June, 2017.

The three-day retreat included talks (and elevator pitches) from fellow postdocs at the institute & invited external speakers:

  • Dr. Simon Reber (Humboldt-Universität, Berlin),
  • Dr. Elvan Boke (Centre for Genomic Regulation, Barcelona),
  • Dr. Selina Wray (University College London),
  • Dr. Adrian Raga (Katholieke Universiteit Leuven),
  • Dr. Olof Idevall-Hagren (Uppsala University);

and talks from our very own: Dr. Jacqueline Tabler and Dr. Christoph Zechner.

The retreat was scientifically enriching and also featured a hiking trip in Bohemian Switzerland, through the sandstone rocks of the Tisá Walls.

2017-06-20 15.48.04     

Lightsheet Micrograph of Zebrafish Embryo.

eLife paper on ZGA timing: activator-repressor competition

Our study demonstrating how repressors (histones) and activators (transcription factors) jointly control transcription in the zebrafish embryo is now available online: Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Joseph et al. eLife (2016)

Competition Model

In this study, lead by Shai Joseph (Vastenhouw Lab) and carried out collaboratively with the Shevchenko and Zaburdaev lab, we could quantitatively address a long-standing question: how is the timing at which transcription starts in embryos controlled?

By a combination of quantitative, molecular, and functional techniques, we found that the two most prominent hypotheses, the "depleted repressor" and the "increasing activator" models, can be unified in a competition model. Here, repressing histones and activating transcription factors go head-to-head competing for access to DNA target sites.

Additionally, the nuclear-to-cytoplasmic ratio - often defined in the number of genomes in an embryo - resurfaced as a key concept, though in the form of a volume ratio between cell nuclei and overall cytoplasm. While the global concentration of not DNA-bound histones did not change at the time of transcription onset, we detected a marked decrease in the concentration of not DNA-bound histones specifically within cell nuclei.

 

BioRxiv logo

BioRxiv: Histone and transcription factor binding competition

A preprint for our latest work is now on BioRxiv: “Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos”. The lead author of this study is Shai, with lots of support from within the Vastenhouw lab as well as the Zaburdaev (MPI-PKS) and Shevchenko labs (MPI-CBG).

You can go and read the preprint here: http://biorxiv.org/content/early/2017/04/13/125716

Segmented Cells

FISHing fish, explained on thenode

Our recent publication is covered in a piece on FISHing fish. Check out the description and a bit of a behind-the-scenes insight into of the paper!

Link to post on thenode

Paper reference:

Stapel, L Carine; Lombardot, Benoit; Broaddus, Coleman; Kainmueller, Dagmar; Jug, Florian; Myers, Eugene W.; Vastenhouw, Nadine L.
Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos
Development, 143, no. 3, pp. 540-546, (2016)
Yuko, Lennart, and LZ1

Short Visit from Yuko Sato

Our collaborator Yuko Sato paid us a short visit from Japan. Yuko is a Kimura Lab Members at the Tokyo Institute of Technology, who develop innovative in vivo imaging approaches targeting post-translational modifications.

During the visit, Yuko and Lennart executed pilot experiments for light sheet-based in vivo imaging. We hope the photo conveys our joy at the last, successful attempt at our experiments. Thank you for the visit!