Book chapter on how to smFISH in zebrafish embryos

Interested in detecting single transcripts from zebrafish embryos? or applying smFISH to your research?

Carine’s chapter in Methods in Molecular Biology is out!

The chapter provides a detailed protocol: from setting up the embryos to cryosection, to smFISH, to image analysis.

You’ll also find a description of the pipeline for membrane segmentation and transcription detection.

Visit Book Chapter at Methods in Molecular Biology

Máté: Submit thesis ✓

Máté reached an important milestone of his PhD studies – thesis submission! High-Five Máté for crunching your way through thesis writing and making it through!

Next up: thesis defense.

PhD defense: Dr. Shai Joseph

 

 

2017.10.30: Congratulations Shai on successfully defending his PhD!

Many thanks to our artistically talented Sabrina for making Shai’s fantastic Doktorhut,

which features past and current lab members partying at “Shai’s Histone Cocktail Bar” atop the hat.

All the best to Shai in his new career path!

 

 

Photo credit (top featured image): IMPRS PhD program

Lennart presents at CBG’s Friday Seminar

20.10.2017: With ~3 years of research experience at CBG up his sleeve, it was now Lennart’s time to take centre stage and present his research to the CBG community at the internal Friday Seminar series. The showcase was followed by social hour with fellow scientists. We capped our Friday off with a belated birthday celebration over delicious burgers.

 

The Vastenhouw Lab goes to Laboratory Animal Science Training

Our lab took part in a one week theory and practical training on “Using Experimental Animals in Research”.
During the course we also got exposed to different kinds of fish: besides our favorite zebrafish, we even performed small experiments with medaka and Tilapia! As is evident from the group picture, we had a lot of fun and would like to thank all the organizers.

Celebrate! Carine’s paper is out & Happy birthday Máté

Cake and champagne to celebrate Máté’s birthday and Carine’s latest achievement: her research on how transcriptional noise is resolved in the embryo is out! Shout out to Christoph Zechner (Center for Systems Biology Dresden), who collaborated with Carine on this story.

Check out the paper in Genes & Development’s August 15th issue: http://genesdev.cshlp.org/content/early/2017/09/13/gad.302935.117

which will feature the cover illustration (below right) from Julia Eichhorn (Media Technologies & Outreach, MPI-CBG).

eLife Paper on Refractive Index Matching in Live Imaging

Microscopes are optimized to give the clearest images, taking great care of all optical components along the light path. This has lead to a situation where the transition between a live sample and the surrounding liquid becomes the biggest imperfection in the optical path.

In this MPI-CBG/MPI-PKS/CSBD collaboration, lead by Tobias Boothe from the Rink lab, we show a simple but highly effective procedure to minimize the optical imperfection at the sample/medium interface. The technique is based on OptiPrep (chemical name: iodixanol), which can be ordered and used in any biological laboratory without any unusual equipment. Our technique worked with no toxicity or other problems in whole animals, primary cells, cell lines, as well as organoids.

Find our paper online with eLife.

Máté at the Zebrafish Meeting in Hungary

July 3rd – 7th, 2017: Máté attended the 10th European Zebrafish Meeting in his hometown, Budapest, and gave a talk in the Genomics/Epigenetics session. The conference brought together the ever-growing zebrafish community (over 500 participants) and covered a wide range of topics. Those who missed it will have to wait until 2020, when the next EZM takes place in Prague.

 

 

Edlyn at MPI-CBG Postdoc Retreat

Edlyn attended the 7th Annual MPI-CBG Postdoc Retreat, which took place in the beautiful and picturesque Tisá, Czech Republic, from 19th to 21st of June, 2017.

The three-day retreat included talks (and elevator pitches) from fellow postdocs at the institute & invited external speakers:

  • Dr. Simon Reber (Humboldt-Universität, Berlin),
  • Dr. Elvan Boke (Centre for Genomic Regulation, Barcelona),
  • Dr. Selina Wray (University College London),
  • Dr. Adrian Raga (Katholieke Universiteit Leuven),
  • Dr. Olof Idevall-Hagren (Uppsala University);

and talks from our very own: Dr. Jacqueline Tabler and Dr. Christoph Zechner.

The retreat was scientifically enriching and also featured a hiking trip in Bohemian Switzerland, through the sandstone rocks of the Tisá Walls.

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