The Vastenhouw Lab goes to Laboratory Animal Science Training

Our lab took part in a one week theory and practical training on “Using Experimental Animals in Research”.
During the course we also got exposed to different kinds of fish: besides our favorite zebrafish, we even performed small experiments with medaka and Tilapia! As is evident from the group picture, we had a lot of fun and would like to thank all the organizers.

Cindy completes Master’s rotation project

Yesterday Cindy presented the results of her Master’s rotation project, which she carried out in our lab over the past couple of months. During the rotation, she worked with Lennart to develop and optimize a new gene excision protocol. Not all details can be revealed yet, but it looks like we can cut long sequences surprisingly early and efficiently!

As was also recognized in Cindy’s exam presentation, she completed a considerable amount of work. She also became a lively part of the lab – so that time passed too fast, again, and her next rotation period is coming up. We wish the best of luck and exciting results!


eLife Paper on Refractive Index Matching in Live Imaging

Microscopes are optimized to give the clearest images, taking great care of all optical components along the light path. This has lead to a situation where the transition between a live sample and the surrounding liquid becomes the biggest imperfection in the optical path.

In this MPI-CBG/MPI-PKS/CSBD collaboration, lead by Tobias Boothe from the Rink lab, we show a simple but highly effective procedure to minimize the optical imperfection at the sample/medium interface. The technique is based on OptiPrep (chemical name: iodixanol), which can be ordered and used in any biological laboratory without any unusual equipment. Our technique worked with no toxicity or other problems in whole animals, primary cells, cell lines, as well as organoids.

Find our paper online with eLife.